Onanong SengvilaipaseuthKoukeo PhommasoneXavier De LamballerieManivanh VongsouvathOoyanong PhonemixayStuart D. BlacksellMayfong MayxaySommay KeomanyPhoutthalavanh SouvannasingPaul N. NewtonAudrey Dubot-PérèsMahosot HospitalÉmergence des Pathologies ViralesUniversité de la Méditerranée Aix-Marseille IINuffield Department of Clinical MedicineMahidol UniversityUniversity of Health SciencesSalavan Provincial Hospital2018-12-212019-03-142018-12-212019-03-142017-01-01PLoS ONE. Vol.12, No.1 (2017)193262032-s2.0-85011066997https://repository.li.mahidol.ac.th/handle/20.500.14594/41619© 2017 Sengvilaipaseuth et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Dengue Duo Rapid Diagnostic Test (SD Dengue RDT) has good specificity and sensitivity for dengue diagnosis in rural tropical areas. In a previous study, using four control sera, we demonstrated that that the diagnostic accuracy of these RDTs remains stable after longterm storage at high temperatures. We extended this study by testing sera from 119 febrile patients collected between July-November 2012 at Salavan Provincial Hospital (southern Laos) with RDTs stored for 6 months at 4°C, 35° and in a hut (miniature traditional house) at Lao ambient temperatures. The dengue NS1 antigen results from RDTs stored at 35°C and in the hut demonstrated 100% agreement with those stored at 4°C. However, lower positive percent agreements, with broad 95%CI, were observed for the tests: IgM, 60% (14.7-94.7) and 40% (5.3-85.3) for RDTs store at 35°C and in the hut, compared to those stored at 4°C, respectively. This study strenghtens the evidence of the robustness of the NS1 antigen detection RDT for the diagnosis of dengue after storage at tropical temperatures.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1371/journal.pone.0170359