Chanon KowasupatBhinyo PanijpanParames LaosinchaiPintip RuenwongsaAmornrat PhongdaraWarapond WannaSaengchan SenapinKornsunee PhiwsaiyaMahidol UniversityPrince of Songkla UniversityThailand National Center for Genetic Engineering and Biotechnology2018-11-092018-11-092014-01-01Meta Gene. Vol.2, No.1 (2014), 83-95221454002-s2.0-84901776503https://repository.li.mahidol.ac.th/handle/20.500.14594/33419In Thailand, there are currently five recognized species members of the bubble-nesting Betta genus, namely Betta splendens, B. smaragdina, B. imbellis, B.mahachaiensis and B. siamorientalis. In 2010, we indicated the possibility, based on COI barcoding evidence, that there might be two additional species, albeit cryptic, related to the type-locality B. smaragdina in someprovinces in the northeast of Thailand. In the present study, after a more extensive survey of the northeast, and phylogenetic analyses based on COI and ITS1 sequences, the B. smaragdina group may be composed of at least 3 cryptic species members. The phylogenetic positions of these B. smaragdina groupmembers in the bubble-nesting bettas' tree together with those of their congeners have been consolidated by better DNA sequence quality and phylogenetic analyses.With a better supported tree, the species statuses of B. siamorientalis and the Cambodian B. smaragdina-like fish, B. stiktos, are also confirmed. © 2013 The Authors.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1016/j.mgene.2013.12.004