Moon Jung KimIl Kwon BaeSeok Hoon JeongSo Hyun KimJae Hoon SongJae Young ChoiSang Sun YoonVisanu ThamlikitkulPo Ren HsuehRohani Md YasinM. K. LalithaKyungwon LeeEulji UniversityYonsei University College of MedicineAsia Pacific Foundation for Infectious Diseases (APFID)SungKyunKwan University, School of MedicineMahidol UniversityNational Taiwan University HospitalInstitute for Medical Research Kuala Lumpur2018-10-192018-10-192013-12-01Journal of Antimicrobial Chemotherapy. Vol.68, No.12 (2013), 2820-282414602091030574532-s2.0-84887517155https://repository.li.mahidol.ac.th/handle/20.500.14594/32070Objectives: To investigate the epidemiological traits of metallo-b-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP). Methods:Atotal of16MPPAclinical isolateswerecollected fromsix Asian countries in2000to2009byANSORP.The MBLgenewasdetected byPCRamplification. The genetic organization of the class1integroncarrying theMBLgene cassettewas investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates. Results: PCR and sequencing experiments detected the blaVIM-2(n=12), blaVIM-3(n=1), blaIMP-6(n=2) and blaIMP-26(n=1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates fromIndia were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298. Conclusions: P. aeruginosa ST235might play a role in dissemination of MBL genes in Asian countries. © The Author 2013. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.Mahidol UniversityMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1093/jac/dkt269