Nuttaporn PimphaUracha RuktanonchaiSuvimol SurassmoPraneet OpanasopitPanya SunintaboonThailand National Science and Technology Development AgencySilpakorn UniversityMahidol University2018-08-242018-08-242007-08-28Proceedings of the 2nd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2007. (2007), 1007-10112-s2.0-34548120157https://repository.li.mahidol.ac.th/handle/20.500.14594/24398The well-defined core-shell amphiphilic nanoparticles consisting of the poly(methyl methacrylate) (PMMA) cores surrounded by the modified chitosan shells were prepared via a surfactant-free emulsion graft-copolymerization of methyl methacrylate from an aqueous modified chitosan solution induced by a small amount of tert-butyl hydroperoxide. The PMMA-chitosan core-shell particles have an average particle diameter of approximately 200 nm and display highly positive surface charges (+70 mV). Transmission electron microscopic (TEM) images clearly reveal a well-defined core-shell morphology of the nanoparticles where the PMMA cores are coated with chitosan shells. The core-shell nanoparticles were investigated as novel carriers for gene delivery. The nanoparticle/plasmid DNA complexes were characterized with respect to their binding affinity, particle size, and zeta-potential. © 2007 IEEE.Mahidol UniversityComputer ScienceEngineeringConference PaperSCOPUS10.1109/NEMS.2007.352188