Kamonpan CharoenkulTaveesak JanetanakitNapawan BunpapongSupanat BoonyapisitsopaRatanaporn TangwangvivatKamol SuwannakarnApiradee TheamboonlersYong PoovorawanAlongkorn AmonsinChulalongkorn UniversityFaculty of Medicine, Siriraj Hospital, Mahidol University2020-10-052020-10-052020-01-01Transboundary and Emerging Diseases. (2020)18651682186516742-s2.0-85089963485https://repository.li.mahidol.ac.th/handle/20.500.14594/59130© 2020 Wiley-VCH GmbH From September 2016 to January 2019, we collected 710 rectal swabs from both healthy and sick dogs from small animal hospitals in 5 provinces of Thailand. The samples were tested for canine rotavirus group A (CRV) by using one-step RT-PCR specific to the VP6 gene. Our results showed that 0.70% (5/710) were positive for CRV. The five CRVs were then characterized by whole-genome sequencing. Our results showed that the genotype of Thai CRVs is G3P[3], which is the predominant genotype reported in dogs. The Thai CRVs posed a novel genetic constellation ‘G3-P[3]-I3-R3-C3-M3-A9-N2-T3-E3-H6’, which has never been reported in CRVs from dogs but has been reported in rotaviruses from humans. Based on phylogenetic analysis, the Thai CRVs are the result of multiple reassortments in which gene segments might have originated from human and bat rotaviruses and suggests the zoonotic potential of the virus.Mahidol UniversityImmunology and MicrobiologyVeterinaryArticleSCOPUS10.1111/tbed.13778