Sakamoto N.Laolerd W.Akeda Y.Sugawara Y.Motooka D.Yamamoto N.Takeuchi D.Shanmugakani R.K.Nishi I.Suzuki M.Shibayama K.Iida T.Santanirand P.Tomono K.Hamada S.Mahidol University2023-07-172023-07-172023-06-01Journal of medical microbiology Vol.72 No.6 (2023)https://repository.li.mahidol.ac.th/handle/20.500.14594/87893Introduction. Carbapenemase-producing Enterobacteriaceae (CPE) have emerged as a global threat to public health and clinical practice.Hypothesis/Gap Statement. In Thailand, reports describing CPEs carrying blaNDM and blaOXA-48-like genes have been increasing recently; however, data on detailed plasmid analysis and temporal shift of sequence type and carbapenemase type are limited.Aim. In this study, we analysed whole-genome sequencing (WGS) data of clinically isolated carbapenemase-producing Klebsiella pneumoniae (CPKP) to reveal the molecular epidemiology of CPKP in a tertiary-care hospital in Bangkok, Thailand.Methodology. Seventy-seven non-duplicated CPKP isolates collected during 2013-2016 were examined for their drug-resistance genes, sequence types and phylogenetic relationships.Results. All the tested isolates possessed carbapenemase gene(s), and the major type of carbapenemase gene in 2014-2015 was blaNDM-1, whereas isolates in 2016 harboured more blaOXA-232 than blaNDM-1. Other carbapenemase gene variants, such as blaNDM-4, blaNDM-5, blaOXA-48, blaOXA-181 and blaIMP-14 were detected in some CPKP isolates. Furthermore, this study revealed that CPKP co-harbouring two genes, blaNDM-1 and blaOXA-232 or blaOXA-181, emerged during this period. Notably, such isolates co-carrying the two carbapenemase genes emerged in three different sequence types, even in a single hospital, and then spread clonally. The WGS of CPKP revealed a temporal shift of the predominant carbapenemase genes from blaNDM-1 to blaOXA-232 along with a variation in other carbapenemase gene types within a span of 4 years.Conclusion. Our findings suggest that a substantial change in CPE types occurred in Thailand and potentially in Southeast Asian countries.Immunology and MicrobiologyArticleSCOPUS10.1099/jmm.0.0017112-s2.0-851639072351473564437294294