Sontana SiritantikornSuthatip JintawornSansanee NoisakranYupin SuputtamongkolDaniel H. ParisStuart D. BlacksellMahidol UniversityThailand National Center for Genetic Engineering and Biotechnology2018-06-112018-06-112012-10-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.106, No.10 (2012), 632-63518783503003592032-s2.0-84865990708https://repository.li.mahidol.ac.th/handle/123456789/14259The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach. © 2012 Royal Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.trstmh.2012.05.004