Thongpradit S.Prasongtanakij S.Srisala S.Chanprasertyothin S.Pasomsub E.Ongphiphadhanakul B.Mahidol University2023-06-202023-06-202022-07-01International Journal of Environmental Research and Public Health Vol.19 No.13 (2022)16617827https://repository.li.mahidol.ac.th/handle/123456789/87285The SARS-CoV-2 virus, which is driving the current COVID-19 epidemic, has been detected in wastewater and is being utilized as a surveillance tool to establish an early warning system to aid in the management and prevention of future pandemics. qPCR is the method usually used to detect SARS-CoV-2 in wastewater. There has been no study using an immunoassay that is less laboratory-intensive than qPCR with a shorter turnaround time. Therefore, we aimed to evaluate the performance of an automated chemiluminescence enzyme immunoassay (CLEIA) for SARS-CoV-2 antigen in wastewater. The CLEIA assay achieved 100% sensitivity and 66.7% specificity in a field-captured wastewater sample compared to the gold standard RT-qPCR. Our early findings suggest that the SARS-CoV-2 antigen can be identified in wastewater samples using an automated CLEIA, reducing the turnaround time and improving the performance of SARS-CoV-2 wastewater monitoring during the pandemic.MedicineArticleSCOPUS10.3390/ijerph191377832-s2.0-851327432921660460135805441