Chusangnin P.Muangthong T.Payuhakrit W.Palipoch S.Suwannalert P.Mahidol University2023-10-082023-10-082023-10-01Anticancer research Vol.43 No.10 (2023) , 4461-4472https://repository.li.mahidol.ac.th/handle/20.500.14594/90349BACKGROUND/AIM: Colorectal cancer (CRC) is strongly associated with altered cadherin adhesion molecules. Oxaliplatin is a standard treatment for CRC, yet high-doses have concerning side effects. In this study, the effects of oxaliplatin and the combination of oxaliplatin with vitamin C on HCT-116 CRC cell migration and invasion were studied through the roles of cellular oxidative stress associated with cadherin molecules. MATERIALS AND METHODS: The cellular assays used in this research were MTT, DCFH-DA, immunofluorescence, and western blotting. Cancer progression was examined using wound healing and Boyden chamber techniques. RESULTS: The results indicate that hydrogen peroxide-induced cellular oxidative stress induced cancer cell migration and invasion. The combined treatment of oxaliplatin with a pro-oxidant concentration of vitamin C resulted in higher toxicity than treatment with oxaliplatin alone. However, treatment with the combination of oxaliplatin and antioxidant concentrations of vitamin C suppressed cancer migration and invasion. Furthermore, the combination treatment increased E-cadherin expression, whereas decreased that of N-cadherin. CONCLUSION: Treatment with the combination of oxaliplatin with vitamin C can inhibit CRC cell growth and decrease cancer cell migration and invasion, via oxidative stress and cadherins.MedicineArticleSCOPUS10.21873/anticanres.166412-s2.0-851728259981791753037772573