Saruth NithitanakoolPimolpan PithayanukulSandrine BourgeoisHatem FessiRapepol BavovadaMahidol UniversityLaboratoire d'Automatique et de Génie des ProcédésUniversite Claude Bernard Lyon 1Chulalongkorn University2018-10-192018-10-192013-06-01Molecules. Vol.18, No.6 (2013), 6504-6520142030492-s2.0-84879665086https://repository.li.mahidol.ac.th/handle/20.500.14594/31532Pectinate gel beads containing Thai mango seed kernel extract (MSKE, cultivar 'Fahlun') were developed and characterised for the purpose of colon-targeted delivery. The MSKE-loaded pectinate beads were prepared using ionotropic gelation with varying pectin-to-MSKE ratios, MSKE concentrations, and concentrations of two cross-linkers (calcium chloride and zinc acetate). The formulated beads were spherical in shape and ranged in size between 1.13 mm and 1.88 mm. Zinc-pectinate (ZPG) beads containing high amounts of MSKE showed complete entrapment efficiency (EE) of MSKE (100%), while calcium-pectinate (CPG) beads demonstrated 70% EE. The in vitro release tests indicated that MSKE-loaded CPG beads were unstable in both simulated gastric medium (SGM) and simulated intestinal medium (SIM), while MSKE-loaded ZPG beads were stable in SIM but unable to prevent the release of MSKE in SGM. The protection of ZPG beads with gastro-resistant capsules (Eudragit® L 100-55) resulted in stability in both SGM and SIM; they disintegrated immediately in simulated colonic medium containing pectinolytic enzymes. MSKE-loaded ZPG beads were stable at 4, 25 and 45 °C during the study period of four months. The present study revealed that ZPG beads in enteric-coated capsules might be a promising carrier for delivering MSKE to the colon. © 2013 by the authors.Mahidol UniversityChemistryMedicineArticleSCOPUS10.3390/molecules18066504