Cameron L. NemethKajohnkiart JanebodinAlex E. YuanJames E. DennisMorayma ReyesDeok Ho KimUniversity of Washington, SeattleMahidol UniversityBenaroya Research Institute at Virginia Mason2018-11-092018-11-092014-01-01Tissue Engineering - Part A. Vol.20, No.21-22 (2014), 2817-28291937335X193733412-s2.0-84909959291https://repository.li.mahidol.ac.th/handle/20.500.14594/33475© 2014, Mary Ann Liebert, Inc. We have examined the effects of surface nanotopography and hyaluronic acid (HA) on in vitro chondrogenesis of dental pulp stem cells (DPSCs). Ultraviolet-assisted capillary force lithography was employed to fabricate well-defined nanostructured scaffolds of composite PEG-GelMA-HA hydrogels that consist of poly(ethylene glycol) dimethacrylate (PEGDMA), methacrylated gelatin (GelMA), and HA. Using this microengineered platform, we first demonstrated that DPSCs formed three-dimensional spheroids, which provide an appropriate environment for in vitro chondrogenic differentiation. We also found that DPSCs cultured on nanopatterned PEG-GelMA-HA scaffolds showed a significant upregulation of the chondrogenic gene markers (Sox9, Alkaline phosphatase, Aggrecan, Procollagen type II, and Procollagen type X), while downregulating the pluripotent stem cell gene, Nanog, and epithelial-mesenchymal genes (Twist, Snail, Slug) compared with tissue culture polystyrene-cultured DPSCs. Immunocytochemistry showed more extensive deposition of collagen type II in DPSCs cultured on the nanopatterned PEG-GelMA-HA scaffolds. These findings suggest that nanotopography and HA provide important cues for promoting chondrogenic differentiation of DPSCs.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringEngineeringMaterials ScienceMedicineArticleSCOPUS10.1089/ten.tea.2013.0614