Eugene AzarkhErin RobinsonSupanee HirunkanokpunBoris AfanasievPattamaporn KittayapongJonathan CarlsonJoe CorsiniColorado State UniversityMahidol UniversityEastern Oregon University2018-07-122018-07-122008-04-25Virology. Vol.374, No.1 (2008), 128-13710960341004268222-s2.0-41649117506https://repository.li.mahidol.ac.th/handle/20.500.14594/19338Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immuno-fluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does NS1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny. © 2007 Elsevier Inc. All rights reserved.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.virol.2007.11.035