Ladawan WasinpiyamongkolSirilaksana PatramoolNatthanej LuplertlopPornapat SurasombatpattanaSouleymane DoucoureFrançois MouchetMartial SévenoFranck RemoueEdith DemettreJean Paul BrizardPatrick JouinDavid G. BironFrédéric ThomasDorothée MisséMahidol UniversityIRD Centre de MontpellierInstitut de Genomique FonctionnelleUniversite Blaise Pascal2018-09-242018-09-242010-05-01Proteomics. Vol.10, No.10 (2010), 1906-191616159861161598532-s2.0-77952375771https://repository.li.mahidol.ac.th/handle/20.500.14594/28727Mosquito-transmitted pathogens pass through the insect's midgut (MG) and salivary gland (SG). What occurs in these organs in response to a blood meal is poorly understood, but identifying the physiological differences between sugar-fed and blood-fed (BF) mosquitoes could shed light on factors important in pathogens transmission. We compared differential protein expression in the MGs and SGs of female Aedes aegypti mosquitoes after a sugar- or blood-based diet. No difference was observed in the MG protein expression levels but certain SG proteins were highly expressed only in BF mosquitoes. In sugar-fed mosquitoes, housekeeping proteins were highly expressed (especially those related to energy metabolism) and actin was up-regulated. The immunofluorescence assay shows that there is no disruption of the SG cytoskeletal after the blood meal. We have generated for the first time the 2-DE profiles of immunogenic Ae. aegypti SG BF-related proteins. These new data could contribute to the understanding of the physiological processes that appear during the blood meal. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1002/pmic.200900626