A serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NP

Nattamon SiboonnanWittawat WiriyaratChompunuch BoonarkartWarunya ChakritbudsabongAnan JongkaewwattanaPilaipan PuthavathanaPrasert AuewarakulOrnpreya SuptawiwatMahidol UniversityNational Center of Genetic Engineering and Biotechnology2018-10-192018-10-192013-06-01Archives of Virology. Vol.158, No.6 (2013), 1151-1157030486082-s2.0-84878536703https://repository.li.mahidol.ac.th/handle/20.500.14594/31909We have generated a temperature-sensitive (ts) mutant from a human isolate of the H5N1 avian influenza virus by classical adaptation in cell culture. After 20 passages at low temperature, the virus showed a ts phenotype. The ts mutant also showed an attenuated phenotype after nasal inoculation in mice. Using reverse genetics, we generated reassortants carrying individual genomic segments of the wild-type and mutant viruses in an A/Puerto Rico/8/34 background, and found that the nucleoprotein (NP) gene could confer the ts phenotype. This mutant NP contains a serine-to-asparagine mutation at position 314 (S314N). The mutant NP protein showed a defect in nuclear localization at high temperature in mammalian cells. © 2013 Springer-Verlag Wien.Mahidol UniversityImmunology and MicrobiologyA serine-to-asparagine mutation at position 314 of H5N1 avian influenza virus NP is a temperature-sensitive mutation that interferes with nuclear localization of NPArticleSCOPUS10.1007/s00705-012-1595-1