Tran N. DangIzumi NakaAreerat Sa-NgasangSurapee AnantapreechaSumalee ChanamaNuanjun WichukchindaPathom SawanpanyalertJintana PatarapotikulNaoyuki TsuchiyaJun OhashiUniversity of TsukubaThailand Ministry of Public HealthThe Food and Drug Administration, Thailand Ministry of Public HealthMahidol University2018-11-092018-11-092014-05-17BMC Medical Genetics. Vol.15, No.1 (2014)147123502-s2.0-84901289187https://repository.li.mahidol.ac.th/handle/123456789/33262Background: Dengue shock syndrome (DSS), a severe life-threatening form of dengue infection, mostly occurs in children. A recent genome wide association study (GWAS) identified two SNPs, rs3132468 of major histocompatibility complex class I polypeptide-related sequence B (MICB) and rs3765524 of phospholipase C, epsilon 1 (PLCE1), associated with DSS in Vietnamese children. In this study, to examine whether an identical association is found in a different population, the association of these two SNPs with DSS was assessed in Thai children with dengue.Methods: The rs3132468 and rs3765524 SNPs were genotyped in 917 Thai children with dengue: 76 patients with DSS and 841 patients with non-DSS. The allele frequencies were compared between DSS and non-DSS groups by one-sided Fisher's exact test. The association of rs3132468 and rs3765524 with the mRNA expression levels of MICB and PLCE1 were assessed in EBV-transformed lymphoblastoid cell lines.Results: The reported DSS-risk alleles were significantly associated with DSS in Thai patients with dengue (one-sided P = 0.0213 and odds ratio [OR] = 1.58 for rs3132468-C and one-sided P = 0.0252 and OR = 1.49 for rs3765524-C). The rs3132468-C allele showed a significant association with lower mRNA level of MICB (P = 0.0267), whereas the rs3765524-C allele did not. These results imply that the MICB molecule may play an important role in the prevention of DSS in dengue infection.Conclusions: Together with previous association studies, we conclude that rs3132468-C at MICB and rs3765524-C at PLCE1 confer risk of DSS in Southeast Asians. © 2014 Dang et al.; licensee BioMed Central Ltd.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1186/1471-2350-15-58