Panithi SukhoGeesien S.A. BoersemaNicole KopsJohan F. LangeJolle KirpensteijnJan Willem HesselinkYvonne M. Bastiaansen-JenniskensFemke VerseijdenDepartement Geneeskunde van Gezelschapsdieren, Universiteit UtrechtErasmus University Medical CenterMahidol UniversityHill's Pet Nutrition Inc.2019-08-232019-08-232018-08-11Journal of Visualized Experiments. Vol.2018, No.138 (2018)1940087X2-s2.0-85054543259https://repository.li.mahidol.ac.th/handle/123456789/45083© 2018 Journal of Visualized Experiments. Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of clinical efficacy, they are until now imperfect solutions. Stem cell therapy using ASC sheets could provide a solution to this problem. ASCs are considered as promising candidates for promoting tissue healing because of their trophic and immunomodulatory properties. Here, we provide methods to produce high-density ASC sheets, that are transplanted onto a colorectal anastomosis in a rat model to reduce the leakage. ASCs formed cell sheets in thermo-responsive culture dishes that could be easily detached. On the day of the transplantation, a partial colectomy with a 5-suture colorectal anastomosis was performed. Animals were immediately transplanted with 1 ASC sheet per rat. ASC sheets adhered spontaneously to the anastomosis without any glue, suture, or any biomaterial. Animal groups were sacrificed 3 and 7 days postoperatively. Compared to transplanted animals, the incidence of anastomotic abscesses and leakage was higher in control animals. In our model, the transplantation of ASC sheets after colorectal anastomosis was successful and associated with a lower leakage rate.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringImmunology and MicrobiologyNeuroscienceArticleSCOPUS10.3791/57213