Charoenchai PuttipanyalearsNakarin KitkumthornSupranee BuranapraditkunSomboon KeelawatApiwat MutiranguraChulalongkorn UniversityMahidol University2018-12-112019-03-142018-12-112019-03-142016-04-01Epigenomics. Vol.8, No.4 (2016), 475-4861750192X175019112-s2.0-84964923704https://repository.li.mahidol.ac.th/handle/20.500.14594/43062© 2016 Future Medicine Ltd. Changes in the methylation level of genes containing LINE-1 alter host gene regulation. Aim: This study demonstrates that paracrine signaling of breast cancer influences the epigenetic regulation of stromal cells. Methods: We proved in vitro and in vivo breast cancer promoted LINE-1 methylation exists exclusively in female stromal cells. Results: Genes containing LINE-1 of breast cancer stromal cells were upregulated. Furthermore, one of the genes, MUC-1, was demonstrated to have expression in plasma cells from the lymph nodes of patients with lymph node metastasis or micrometastasis. Conclusion: Breast cancer sends a paracrine signal to stroma cells causing LINE-1 epigenetic regulation. Moreover, the regulated genes in stroma cells are potential biomarkers for detecting breast cancer micrometastasis.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.2217/epi-2015-0007