Ornpreya SuptawiwatChompunuch BoonarkartPrasert AuewarakulMahidol University2018-09-242018-09-242010-11-01Archives of Virology. Vol.155, No.11 (2010), 1789-1795030486082-s2.0-78149411486https://repository.li.mahidol.ac.th/handle/123456789/29179We have previously described a cis enhancing sequence (CES) in the gp41 region of the HIV-1 env gene. It could enhance HIV-1 Gag expression via both rev-dependent and CTE (constitutive transport element)-dependent pathways. We identified a functionally important and conserved region in the CES that contained a predicted binding sequence for an SR protein, ASF/SF2. We show here that ASF/SF2 bound to this sequence in an electrophoretic mobility shift assay and that the putative ASF/SF2-binding sequence was required for the enhancement of Gag expression by CES and might play a role in HIV-1 posttranscriptional regulation. © 2010 Springer-Verlag.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1007/s00705-010-0765-2