Vanaporn WuthiekanunPremjit AmornchaiSayan LanglaNicholas J. WhiteNicholas P.J. DayDirek LimmathurotsakulSharon J. PeacockMahidol UniversityUniversity of Oxford2018-11-232018-11-232015-01-01American Journal of Tropical Medicine and Hygiene. Vol.93, No.2 (2015), 241-243000296372-s2.0-84939223945https://repository.li.mahidol.ac.th/handle/123456789/36152© 2015 by The American Society of Tropical Medicine and Hygiene. Leptospira Vanaporn Wuthiekanun (LVW) agar was used to develop a disk diffusion assay for Leptospira spp. Ten pathogenic Leptospira isolates were tested, all of which were susceptible to 17 antimicrobial agents (amoxicillin/ clavulanic acid, amoxicillin, azithromycin, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, clindamycin, doripenem, doxycycline, gentamicin, linezolid, nitrofurantoin, penicillin, piperacillin/tazobactam, and tetracycline). All 10 isolates had no zone of growth inhibition for four antimicrobials (fosfomycin, nalidixic acid, rifampicin, and trimethoprim/ sulfamethoxazole). Of the ten Leptospira, seven had a growth inhibition zone of 21 mm for aztreonam, the zone diameter susceptibility break point for Enterobacteriaceae. This assay could find utility as a simple screening method during the epidemiological surveillance of antimicrobial resistance in Leptospira spp.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.4269/ajtmh.15-0180