Suvit LimawongpraneeDarawan WanachiwanawinKulkanya ChokephaibulkitJerawan OnchrotchanakunPunpop LertlaituanSuphaluck WankhomMahidol University2018-08-242018-08-242007-09-01Southeast Asian Journal of Tropical Medicine and Public Health. Vol.38, No.5 (2007), 892-896012515622-s2.0-35349031020https://repository.li.mahidol.ac.th/handle/123456789/24752One hundred and five samples of gastric washes were obtained from 52 pediatric patients. Eleven of the 105 samples (10%) gave positive results using immunofluorescence antibody test (IFA) for Pneumocystis jirovecii. Single-step polymerase chain reaction (PCR) produced 13 % (14 samples), whereas detection by nested PCR was increased to 65 samples (62%). Moderate agreement (kappa = 0.5) was found between test results of IFA and single-step PCR, but no agreement was found between the results of IFA and nested PCR (kappa = 0.1).Mahidol UniversityMedicineArticleSCOPUS