Rattana PhadungrakwittayaSirikul ChotewuttakornManoon PiwtongOnusa ThamsermsangTawee LaohapandPravit AkarasereenontFaculty of Medicine, Siriraj Hospital, Mahidol University2020-01-272020-01-272019-01-01Siriraj Medical Journal. Vol.71, No.3 (2019), 240-245222880822-s2.0-85067197770https://repository.li.mahidol.ac.th/handle/20.500.14594/52107© 2019, Siriraj Medical Journal. Objective: To identify active compounds and establish the chemical fingerprint of Artemisia annua L. for the quality control. Methods: Thin-layer chromatography (TLC) conditions were developed to screen for 2 common flavonoids (apigenin and luteolin). Three mobile phases were used to isolate these flavonoids in 80% ethanolic extract of A. annua. Hexane: ethyl acetate: acetic acid (31:14:5, v/v) and toluene: 1,4-dioxane: acetic acid (90:25:4, v/v) were used in normal phase TLC (NP-TLC), and 5.5% formic acid in water: methanol (50:50, v/v) were used in reverse phase TLC (RP-TLC). Chromatograms were visualized under visible light after spraying with Fast Blue B Salt. Apigenin and luteolin bands were checked by comparing their Rf values and UV-Vis absorption spectra with reference markers. Results: Apigenin and luteolin were simultaneously detected with good specificity in RP-TLC condition, while only apigenin was detected in NP-TLC condition. Apigenin band intensity was higher than luteolin band intensity in both conditions. Conclusion: This knowledge can be applied to the development of quality control assessments to ensure product efficacy and consistency.Mahidol UniversityMedicineArticleSCOPUS10.33192/Smj.2019.37