Boonrat ChantongSupim WongtongtairPunnee NusuetrongUthai SotanaphunChaiyo ChaichantipyuthDuangdeun MeksuriyenMahidol UniversityChulalongkorn UniversitySrinakharinwirot UniversitySilpakorn University2018-09-132018-09-132009-03-01Planta Medica. Vol.75, No.4 (2009), 346-35014390221003209432-s2.0-65649111135https://repository.li.mahidol.ac.th/handle/20.500.14594/27277The aim of the present study was to investigate the stability of barakol, an anxiolytic constituent extracted from leaves of Senna siamea (Lam.) Irwin & Barneby (syn. Cassia siamea Lam.), under the International Conference on Harmonisation suggested conditions using HPLC with photodiode array detection. Extensive degradation of barakol was found to occur under alkaline conditions through base-catalyzed hydrolysis. Mild degradation of barakol was observed under thermal and oxidative stress while it was stable under acidic conditions. The reaction rate constants (kobs) of barakol degradation under alkaline conditions at pHs 12 and 13 were 3.0x10-5 and 9.6x10 -3 min-1, respectively. The activation energy according to the Arrhenius plotwas calculated to be 26.9 ± 3.3 kcal/mol at pH 13 and temperatures between 12 and 51 °C. The major degradation product of barakol under both alkaline and thermal stress conditions was characterized by LC-MS and NMR as cassiachromone. © Georg Thieme Verlag KG Stuttgart.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1055/s-0028-1112206