Saovaros SvastiShizuka MasakiTipparat PenglongYasunobu AbePranee WinichagoonSuthat FucharoenTsukuru UmemuraMahidol UniversityKyushu University2018-09-242018-09-242010-10-01Annals of Hematology. Vol.89, No.10 (2010), 953-958093955552-s2.0-77956685615https://repository.li.mahidol.ac.th/handle/20.500.14594/29503MicroRNAs (miRNAs) are negative regulators of gene expression that play an important role in hematopoiesis. Thalassemia, a defective globin synthesis leading to precipitate of excess unbound globins in red blood cell precursors, results in defective erythroid precursors and ineffective erythropoiesis. Expression pattern of miR-451, an erythroid-specific miRNA, was analyzed during differentiation of erythroid progenitors derived from normal and thalassemic peripheral blood CD34-positive cells, after 14 days of culture. A biphasic expression with transient up-regulation of miRNA-451 on day 3 of cultures was observed during thalassemic erythroid differentiation. In contrast, the expression pattern of the miR-451 in erythroid cells obtained from the other extravascular hemolytic anemia, i.e., hereditary spherocytosis patients showed no transient up-regulation of miR-451 on day 3 of cultures. Our results suggest that early erythroid progenitors in β-thalassemia have a dysregulated miRNA-451 expression program, and analysis of microRNA is a relevant approach to determine abnormalities of erythropoiesis. © 2010 Springer-Verlag.Mahidol UniversityMedicineArticleSCOPUS10.1007/s00277-010-0980-7