Warunya ChakritbudsabongLadawan SariyaSirikron PamonsupornvichitRassmeepen PronarkngverSomjit ChaiwattanarungruengpaisanJoao N. FerreiraPiyathip SetthawongPraopilas PhakdeedindanMongkol TechakumphuTheerawat TharasanitSasitorn RungarunlertMahidol UniversityNational University of Singapore, Faculty of DentistryChulalongkorn University2018-12-212019-03-142018-12-212019-03-142017-10-01Stem Cell Research. Vol.24, (2017), 21-2418767753187350612-s2.0-85029441927https://repository.li.mahidol.ac.th/handle/20.500.14594/41752© 2017 The Authors Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1016/j.scr.2017.08.005