Nitat SookrungJintarat ChoopongWatee SeesuayNitaya IndrawattanaWanpen ChaicumpaAnchalee TungtrongchitrMahidol University2018-12-112019-03-142018-12-112019-03-142016-03-01Asian Pacific Journal of Allergy and Immunology. Vol.34, No.1 (2016), 51-58222886940125877X2-s2.0-84962508002https://repository.li.mahidol.ac.th/handle/20.500.14594/40860© 2016, Allergy and Immunology Society of Thailand. All rights reserved. Background and objective: Natural allergenic extracts using for diagnosis and immunotherapy may have batch-to-batch variations and contaminations with unrefined allergens or nonallergenic components. Thus, recombinant allergen is believed to overcome these shortcomings. In this study, native and recombinant allergens of group 1 and 2 of Dermatophagoides mites were produced and their allergenicities were compared. Methods: Native allergens were prepared by MAb affinity chromatography. All recombinant allergens were produced in E. coli expression system. IgE reactivities of these allergens were determined by IgE-ELISA. Results: The native and recombinant Der p 1, Der p 2, Der f 1, Der f 2 had molecular weights of approximately 25, 15, 25 and 15 kDa, respectively. IgE reactivities of nDer p 1, nDer f 1, rDer p 1 and rDer f 1 were 96.67%, 90%, 43.33% and 46.67%, respectively. Allergenicities of nDer p 2, nDer f 2, rDer p 2 and rDer f 2 were 86.67%, 96.43%, 76.67% and 89.29%, respectively. The findings indicated that recombinant group-1 products were minor allergens which revealed no correlation with their native forms. In contrast, recombinant group-2 allergens were major allergens and showed a significant correlation to their native allergens. Conclusion: We successfully produced native and recombinant group-1 and group-2 allergens. According to their allergenicities, recombinant Der p 2 and rDer f 2 have potential to replace native allergen in diagnostic and therapeutic extracts. Moreover, they can employ as a standard reagent to measure the amount of group 2 allergen in the environment by sandwich-ELISA and utilise this as an immunogen for MAb production.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.12932/AP0670.34.1.2016