Pattsarun CheawchanlertfaSawannee SutheeworapongPiroon JenjaroenpunThidathip WongsurawatIntawat NookaewSupapon CheevadhanarakAkihiko KosugiPatthra PasonRattiya WaeonukulKhanok RatanakhanokchaiChakrit TachaapaikoonUniversity of Arkansas for Medical SciencesJapan International Research Center for Agricultural SciencesFaculty of Medicine, Siriraj Hospital, Mahidol UniversityKing Mongkut s University of Technology Thonburi2020-12-282020-12-282020-11-16PeerJ. Vol.8, (2020)216783592-s2.0-85096295727https://repository.li.mahidol.ac.th/handle/123456789/60347Copyright 2020 Cheawchanlertfa et al. Distributed under Creative Commons CC-BY 4.0 Background. Cassava pulp is a promising starch-based biomasses, which consists of residual starch granules entrapped in plant cell wall containing non-starch polysaccharides, cellulose and hemicellulose. Strain CT4T, a novel mesophilic anaerobic bacterium isolated from soil collected from a cassava pulp landfill, has a strong ability to degrade polysaccharides in cassava pulp. This study explored a rarely described species within the genus Clostridium that possessed a group of cassava pulp-degrading enzymes. Methods. A novel mesophilic anaerobic bacterium, the strain CT4T, was identified based on phylogenetic, genomic, phenotypic and chemotaxonomic analysis. The complete genome of the strain CT4T was obtained following whole-genome sequencing, assembly and annotation using both Illumina and Oxford Nanopore Technology (ONT) platforms. Results. Analysis based on the 16S rRNA gene sequence indicated that strain CT4T is a species of genus Clostridium. Analysis of the whole-genome average amino acid identity (AAI) of strain CT4T and the other 665 closely related species of the genus Clostridium revealed a separated strain CT4T from the others. The results revealed that the genome consisted of a 6.3 Mb circular chromosome with 5,664 protein-coding sequences. Genome analysis result of strain CT4T revealed that it contained a set of genes encoding amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. A comparative genomic analysis of strain CT4T with closely related species with available genomic information, C. amylolyticum SW408T, showed that strain CT4T contained more genes encoding cassava pulp-degrading enzymes, which comprised a complex mixture of amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes.Mahidol UniversityAgricultural and Biological SciencesBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.7717/peerj.10343