Noppamas SoonthornchareonnonKhanit SuwanboriruxRapepol BavovadaChamnan PatarapanichJohn M. CassadyChulalongkorn UniversityOhio State UniversityMahidol University2018-09-072018-09-071999-10-01Journal of Natural Products. Vol.62, No.10 (1999), 1390-1394016338642-s2.0-0032749521https://repository.li.mahidol.ac.th/handle/20.500.14594/25277Guided by brine shrimp toxicity and human tumor cell toxicity, fractionation of the alcoholic extract from the stem bark of Goniothalamus marcanii led to the isolation of four new 1-azaanthraquinones: marcanines B (3), C (4), D (5), and E (6), along with two known derivatives: marcanine A and dielsiquinone. A new 5-hydroxy-3-amino-2-aceto-1,4-naphthoquinone (7), a possible 1-azaanthraquinone biosynthetic precursor, was also isolated. The structures of the compounds were elucidated by spectroscopic analyses, mainly 1D and 2D NMR techniques (1H, 13C, NOEDS, COSY, HMQC, and HMBC), as well as comparison with literature data. All the compounds except 6 were evaluated for cytotoxic activity. They exhibited significant cytotoxicity against several human tumor cell lines, A-549, HT-29, MCF7, RPMI, and U251 with the ED50 in the range of 0.04-3.03 μM.Mahidol UniversityAgricultural and Biological SciencesChemistryPharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1021/np990197c