Karen GaudinTina KaussAlexandra GaubertVincent ViaudJean Pierre DubostPiero OlliaroNicholas J. WhitePascal MilletDeveloppements Analytiques et Pharmaceutiques Appliques aux Maladies Negligees et Aux ContrefaconsOrganisation Mondiale de la SanteNuffield Department of Clinical MedicineMahidol University2018-05-032018-05-032011-11-01Analytical Letters. Vol.44, No.16 (2011), 2732-27431532236X000327192-s2.0-84857770029https://repository.li.mahidol.ac.th/handle/20.500.14594/11448Chromatographic parameter assessments for RP-HPLC-UV method development for the simultaneous analysis of artemether and azithromycin for the pharmaceutical analysis of a rectal coformulation currently under development for the treatment of malaria infected children. Using methanol based mobile phase for the analysis of both artemether and azithromycin provided a more robust method in terms of resolution and peak symmetry. The method validated for suppository used 80% methanol and 20% phosphate buffer 15 mM at pH 9. The UV detection was at 210 nm. The accuracy profiles indicated a method validation between 80-120% for both active pharmaceutical ingredients. The preparation process of the suppository was validated based on theoretical values of artemether and azithromycin present in the formulation; active pharmaceutical ingredients were homogenously distributed within the suppository. © 2011 Copyright Taylor and Francis Group, LLC.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryMedicineArticleSCOPUS10.1080/00032719.2011.553014