L. SratthaphutN. RuangwisesSilpakorn UniversityMahidol University2018-06-112018-06-112012-01-01Procedia Engineering. Vol.32, (2012), 225-231187770582-s2.0-84880845731https://repository.li.mahidol.ac.th/handle/20.500.14594/14179This study illustrated the genetic algorithms (GA) is useful tool in wavelength selection for the spectrophotometric determination of vitamin B 12 in pharmaceutical tablets by partial least-squares calibration procedure (PLS). Because of interference in the spectra, a training set of the mixtures of vitamin B 12 and tablet excipients was built to be used as calibration set for modeling. The absorption spectra of all solutions were recorded each 1 nm in the range 354-600 nm, using 0.1N hydrochloric acid as solvent. A validation set was employed to verify the models and a commercial sample was analysed. GA-PLS model leads to 86% improvement of predictive ability for vitamin B 12 , when comparing with full spectrum PLS model. It is concluded that only some variables in GA-PLS modeling are relevant for prediction and removing the non-relevant variables can improve the predictive capability. © 2010 Published by Elsevier Ltd.Mahidol UniversityEngineeringConference PaperSCOPUS10.1016/j.proeng.2012.01.1261