Brom J.A.Samsri S.Petrikis R.G.Parnham S.Pielak G.J.Mahidol University2023-09-052023-09-052023-01-01Biomolecular NMR Assignments (2023)18742718https://repository.li.mahidol.ac.th/handle/123456789/89370Adenylate kinase reversibly catalyzes the conversion of ATP plus AMP to two ADPs. This essential catalyst is present in every cell, and the Escherichia coli protein is often employed as a model enzyme. Our aim is to use the E. coli enzyme to understand dry protein structure and protection. Here, we report the expression, purification, steady-state assay, NMR conditions and 1H, 13C, 15N backbone resonance NMR assignments of its C77S variant. These data will also help others utilize this prototypical enzyme.Biochemistry, Genetics and Molecular Biology<sup>1</sup>H, <sup>13</sup>C, <sup>15</sup>N backbone resonance assignment of Escherichia coli adenylate kinaseArticleSCOPUS10.1007/s12104-023-10147-12-s2.0-851690933251874270X