Atefeh KhakpoorMingkwan PanyasrivanitNitwara WikanDuncan R. SmithMahidol University2018-09-132018-09-132009-06-29Journal of General Virology. Vol.90, No.5 (2009), 1093-110314652099002213172-s2.0-67449086380https://repository.li.mahidol.ac.th/handle/20.500.14594/27697We have recently proposed that amphisomes act as a site for translation and replication of dengue virus (DENV)-2 and that DENV-2 entry and replication are linked through an ongoing association with membranes of an endosomal-autophagosomal lineage. In this report, we present the results of an investigation into the interaction between DENV-3 and the autophagy machinery. Critically, treatment with the lysosomal fusion inhibitor L-asparagine differentiated the interaction of DENV-3 from that of DENV-2. Inhibition of fusion of autophagosomes and amphisomes with lysosomes resulted in decreased DENV-3 production, implying a role for the autophagolysosome in the DENV-3 life cycle. Evidence based upon the co-localization of LC3 and cathepsin D with double stranded RNA and NS1 protein, as assessed by confocal microscopy, support a model in which DENV-3 interacts with both amphisomes and autophagolysosomes. These results demonstrate that the interactions between DENV and the host cell autophagy machinery are complex and may be determined in part by virus-encoded factors. © 2009 SGM.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1099/vir.0.007914-0