Natpapas WiriyachaipornNisachon ApiwatWarangkana ChantimaTararaj DharakulThailand National Science and Technology Development AgencyFaculty of Medicine, Thammasat University2018-06-112018-06-112012-12-01IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. (2012), 36-4021596972215969642-s2.0-84880212034https://repository.li.mahidol.ac.th/handle/20.500.14594/13568A combined gold nanoparticle (GNP)-monoclonal antibody (MAb) conjugate was generated for a direct influenza A antigen detection using lateral flow assay (LF) format. The combination of GNP-MAb conjugate targeting both nucleoprotein (NP) and matrix protein (M) allows the detection of multiple influenza A proteins. The LF system consisting of combined GNP-MAb conjugate showed an improved sensitivity, compared to the LF system containing single GNP-MAb conjugate targeting only one target protein, either NP or M. This was illustrated by the 2- to 260-fold lower limit of detection of the combined GNP-MAb conjugate LF than the single GNP-MAb conjugate LF. As such, the combined GNP-MAb conjugate LF could provide the basis for developing a direct influenza A antigen detection for rapid influenza A diagnosis. © 2012 IEEE.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyEngineeringMedicineConference PaperSCOPUS10.1109/NANOMED.2012.6509123