Ampai TanganuchitcharnchaiLee SmytheMichael DohntRudy HartskeerlManivanh VongsouvathViengmone DavongOlay LattanaPaul N. NewtonStuart D. BlacksellMahidol UniversityWorld Health Organization, AustraliaRoyal Tropical Institute - KITMahosot HospitalUniversity of Oxford2018-06-112018-06-112012-09-01Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.106, No.9 (2012), 563-56618783503003592032-s2.0-84864819561https://repository.li.mahidol.ac.th/handle/20.500.14594/14278The diagnostic utility of the Standard Diagnostics . Leptospira IgM ELISA for detection of acute leptospirosis was assessed in febrile adults admitted in Vientiane, Laos. Using the cut-off suggested by the manufacturer [optical density (OD) ≥0.75], the assay demonstrated limited diagnostic capacity with a sensitivity of 95% and a specificity of 41% compared with the . Leptospira microscopic agglutination test, which is the serological gold standard. However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%). © 2012 Royal Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.trstmh.2012.06.002