Bungonsiri IntraAnja GreuleAndreas BechtholdJirayut EuanorasetrThomas PaululatWatanalai PanbangredMahidol UniversityUniversität Freiburg im BreisgauUniversitat Siegen2018-12-112019-03-142018-12-112019-03-142016-06-29Journal of Agricultural and Food Chemistry. Vol.64, No.25 (2016), 5171-517915205118002185612-s2.0-84976628483https://repository.li.mahidol.ac.th/handle/123456789/43065© 2016 American Chemical Society. Two new polyene macrolactone antibiotics, thailandins A, 1, and B, 2, were isolated from the fermentation broth of rhizosphere soil-associated Actinokineospora bangkokensis strain 44EHWT. The new compounds from this strain were purified using semipreparative HPLC and Sephadex LH-20 gel filtration while following an antifungal activity guided fractionation. Their structures were elucidated through spectroscopic techniques including UV, HR-ESI-MS, and NMR. These compounds demonstrated broad spectrum antifungal activity against fungi causing anthracnose disease (Colletotrichum gloeosporioides DoA d0762, Colletotrichum gloeosporiodes DoA c1060, and Colletotrichum capsici DoA c1511) as well as pathogenic yeasts (Candida albicans MT 2013/1, Candida parasilopsis DKMU 434, and Cryptococcus neoformans MT 2013/2) with minimum inhibitory concentrations ranging between 16 and 32 μg/mL. This is the first report of polyene antibiotics produced by Actinokineospora species as bioactive compounds against anthracnose fungi and pathogenic yeast strains.Mahidol UniversityAgricultural and Biological SciencesChemistryArticleSCOPUS10.1021/acs.jafc.6b01119