Krongyut C.Wiriya N.Saiyasombat W.Chansaenpak K.Sripattanakul S.Kamkaew A.Lai R.Y.Mahidol University2024-11-282024-11-282024-01-01ChemBioChem (2024)14394227https://repository.li.mahidol.ac.th/handle/123456789/1021974-Hydroxyisochroman-1-ones belong to the class of the secondary metabolite 3,4-dihydroisocoumarins. They exhibit a wide range of biological activities. These compounds can be synthesized through halocyclization using hypervalent iodine species or N-bromosuccinimide, followed by hydrolysis. Nonetheless, the reactions required specific conditions and generated toxic byproducts. In this study, Curvularia inaequalis vanadium chloroperoxidase (CiVCPO) catalyzed the chemoenzymatic cyclization of 2-vinylbenzoic acids with different electron-donating groups (1 a–1 e) to produce good yields of 4-hydroxyisochroman-1-ones (3 a–3 e) by adding KBr and H2O2 in citrate buffer (pH 5). The reaction mixture contained 30 % DMSO to improve substrate solubility without enzyme activity loss. The condition is more environmentally friendly than chemical methods. Therefore, it offers an alternative approach for synthesizing 4-hydroxyisochroman-1-ones.ChemistryBiochemistry, Genetics and Molecular BiologyArticleSCOPUS10.1002/cbic.2024006972-s2.0-852098153401439763339489699