Mark R. LilesLynn L. WilliamsonJitsupang RodbumrerVigdis TorsvikRobert M. GoodmanJo HandelsmanAuburn UniversityUniversity of Wisconsin MadisonMahidol UniversityUniversitetet i BergenSchool of Environmental and Biological SciencesRutgers, The State University of New Jersey2018-07-122018-07-122008-05-01Applied and Environmental Microbiology. Vol.74, No.10 (2008), 3302-3305009922402-s2.0-44249127260https://repository.li.mahidol.ac.th/handle/20.500.14594/18935We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries. Copyright © 2008, American Society for Microbiology. All Rights Reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyEnvironmental ScienceImmunology and MicrobiologyArticleSCOPUS10.1128/AEM.02630-07