Chairat TantrawatpanPewpan M. IntapanPenchom JanwanOranuch SanpoolViraphong LulitanondChutatip SrichantaratsameeWitthaya AnamnartWanchai MaleewongKhon Kaen UniversityFaculty of Medicine, Thammasat UniversityMahidol UniversityWalailak University2018-10-192018-10-192013-06-01Parasitology International. Vol.62, No.3 (2013), 341-34518730329138357692-s2.0-84876327210https://repository.li.mahidol.ac.th/handle/20.500.14594/31918DNA pyrosequencing for PCR amplicons is an attractive strategy for the identification of microorganisms because of its short time performance for large number of samples. In this study, the primers targeting the fragment of ITS2 region of nuclear ribosomal RNA gene were newly developed for pyrosequencing-based identification of 6 Paragonimus species, Paragonimus bangkokensis, Paragonimus harinasutai, Paragonimus heterotremus, Paragonimus macrorchis, Paragonimus siamensis and Paragonimus westermani. Pyrosequencing determination of 39 nucleotides of partial ITS2 region could discriminate 6 Paragonimus species, and could also detect intra-species genetic variation of P. macrorchis. This DNA pyrosequencing-based identification can be a valuable tool to improve species-level identification of Paragonimus in the endemic areas. © 2012 Elsevier Ireland Ltd.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS10.1016/j.parint.2012.11.008