Kittipong WerawattanametinVerapong PodimuangApichart SuksamrarnMahidol UniversityKhon Kaen UniversityRamkhamhaeng University2018-02-272018-02-271986-01-01Journal of Natural Products. Vol.49, No.2 (1986), 365-36615206025016338642-s2.0-0022490163https://repository.li.mahidol.ac.th/handle/20.500.14594/9680Vitex glabrata R. Br. (Verbenaceae) is a tree locally known as “Kai Nao” (1). The bark and root have reportedly been used as astringents. The bark has also been claimed to be an anthelmintic and a remedy for gastro-intestinal disorders (2,3). We report here the isolation and identification of two ecdysteroids: 20-hydroxyecdysone (4) and 1 la,20-dihydroxyecdysone (turkesterone) (5) from the bark of V. glabrata. Continuous liquid-liquid extraction provides a convenient and effective isolation of 20-hydroxyecdysone in high yield. The occurrences of 20-hydroxyecdysone and related ecdysteroids in Vitex megapotamica have been reported (6–8). To our knowledge, this is the first report of 11α,20-dihydroxyecdysone in Verbenaceae (9). © 1986, American Chemical Society. All rights reserved.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemistryMedicinePharmacology, Toxicology and PharmaceuticsArticleSCOPUS10.1021/np50044a041