La ongthong VajrabhayaSuwanna KorsuwannawongClaudia BoslGottifried SchmalzMahidol UniversityUniversitat Regensburg2018-09-132018-09-132009-03-01Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology. Vol.107, No.3 (2009)107921042-s2.0-59649104998https://repository.li.mahidol.ac.th/handle/20.500.14594/27517Objective: This study evaluated the cytotoxicity of 3 self-etching bonding systems: Optibond Solo Plus SE primer, Xeno III, and i Bond. Study design: The test materials were applied on the dentine discs of dentine barrier models in the same way as in the clinical procedures recommended by each manufacturer. 3-D cell culture of Bovine pulp-derived cells transfected with Simian virus 40 Large T antigen and perfusion condition were conducted in this experiment. Cell viability after exposure to the bonding agents was determined by dimethylthiazolediphenyltetrazolium bromide (MTT) assay. Results: The results revealed that cell survival with the above-mentioned bonding agents was 99.66%, 72.59%, and 10.65%, respectively. i Bond is the most toxic material (P < .05). Xeno III is less toxic than i Bond but more toxic than Optibond Solo Plus SE primer (P < .05). Conclusions: Among the 3 test self-etching bonding systems, Optibond Solo Plus SE primer should be selected in cases where the remaining dentin above pulp tissue is 0.5 mm or less. © 2009 Mosby, Inc. All rights reserved.Mahidol UniversityDentistryMedicineArticleSCOPUS10.1016/j.tripleo.2008.11.008