Apichart NgernsoungnernPiyada NgernsoungnernScott KavanaughStacia A. SowerPrasert SobhonPrapee SretarugsaMahidol UniversityUniversity of New Hampshire Durham2018-07-122018-07-122008-03-01Invertebrate Neuroscience. Vol.8, No.1 (2008), 49-57135425162-s2.0-40149095367https://repository.li.mahidol.ac.th/handle/123456789/19855In the present study, we demonstrated the existence of GnRH-like peptides in the central nervous system (CNS) and ovary of the giant freshwater prawn, Macrobrachium rosenbergii using immunocytochemistry. The immunoreactivity (ir) of lamprey (l) GnRH-III was detected in the soma of medium-sized neurons located in neuronal cluster number 11 in the middle part of supraesophageal ganglion (deutocerebrum), whereas ir-octopus (oct) GnRH was observed in the soma of both medium-sized and large-sized neurons in thoracic ganglia, as well as in the fibers innervating the other medium-sized and large-sized neuronal cell bodies in the thoracic ganglia. In addition, ir-lGnRH-I was observed in the cytoplasm of late previtellogenic oocyte and early vitellogenic oocyte. These data suggest that M. rosenbergii contain at least three isoforms of GnRH: two GnRH isoforms closely related to lGnRH-III and octGnRH in the CNS, whereas another isoform, closely related to lGnRH-I, was localized in the ovary. This finding provides supporting data that ir-GnRH-like peptide(s) may exist in this decapod crustacean. © 2008 Springer-Verlag.Mahidol UniversityNeuroscienceArticleSCOPUS10.1007/s10158-008-0067-5