Kanokpan WongprasertTawut RudtanatipJantana PraiboonMahidol UniversityKasetsart University2018-11-092018-11-092014-01-01Fish and Shellfish Immunology. Vol.36, No.1 (2014), 52-6010959947105046482-s2.0-84891154542https://repository.li.mahidol.ac.th/handle/123456789/33099Sulfated galactans (SG) were isolated from the red seaweed Gracilaria fisheri (G.fisheri). Chemical analysis revealed SG contains sulfate (12.7%) and total carbohydrate (42.2%) with an estimated molecular mass of 100kDa. Structure analysis by NMR and FT-IR spectroscopy revealed that SG is a complex structure with a linear backbone of alternating 3-linked β-d-galactopyranose and 4-linked 3,6-anhydrogalactose units with partial 6-O-methylate-β-d-galactopyranose and with sulfation occurring on C4 of d-galactopyranose and C6 of l-galactopyranose units. SG treatment enhanced immune parameters including total haemocytes, phenoloxidase activity, superoxide anions and superoxide dismutase in shrimp Penaeus monodon. Shrimp fed with Artemia salina enriched with SG (100 and 200μgml-1) and inoculated with white spot syndrome virus (WSSV) showed a significantly lower mortality rate and lower viral VP 28 amplification and expression than control. The results suggest that SG from G.fisheri exhibits immune stimulatory and antiviral activities that could protect P.monodon from WSSV infection. © 2013 Elsevier Ltd.Mahidol UniversityAgricultural and Biological SciencesEnvironmental ScienceArticleSCOPUS10.1016/j.fsi.2013.10.010