Bunpote SiridechadilokMethee GomutsukhavadeeThunyarat SawaengpolSutha SangiambutChunya PuttikhuntKwanrutai Chin-inmanuPrapat SuriyapholPrida MalasitGavin ScreatonJuthathip MongkolsapayaThailand National Center for Genetic Engineering and BiotechnologyMahidol UniversityImperial College London2018-10-192018-10-192013-12-01Journal of Virology. Vol.87, No.23 (2013), 12667-12674109855140022538X2-s2.0-84887207401https://repository.li.mahidol.ac.th/handle/123456789/31826Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses. © 2013, American Society for Microbiology.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1128/JVI.02261-13