Sutthidech PreecharramPornpimol JearranaiprepameSakda DaduangYosapong TemsiripongTheerasak SomdeeTamo FukamizoJisnuson SvastiTomohiro ArakiSompong ThammasirirakKhon Kaen UniversitySriracha Moda Farm Chon BuriKindai UniversityMahidol UniversityTokai University2018-09-242018-09-242010-06-01Animal Science Journal. Vol.81, No.3 (2010), 393-40117400929134439412-s2.0-77953883209https://repository.li.mahidol.ac.th/handle/20.500.14594/28498An antibacterial compound from crocodile blood was partially purified and functionally characterised. The freshwater crocodile (Crocodylus siamensis) plasma with antibacterial activity was partially purified by using a centrifugal concentrator and reverse phase high powered liquid chromatography, and designated as crocosin. Crocosin exhibits antibacterial activity toward Salmonella typhi and Staphylococcus aureus. Crocosin is thermostable and resistant to pronase digestion. The structure of crocosin analyzed by mass spectrometry contains repeating units of 94 and 136 m/z. Scanning electron microscopy indicates that crocosin probably penetrates progressively into cytoplasm space, perturbing and damaging bacterial membranes. Crocosin may provide an early defense mechanism toward bacterial infection in freshwater. © 2010 The Authors. Journal compilation © 2010 Japanese Society of Animal Science.Mahidol UniversityAgricultural and Biological SciencesArticleSCOPUS10.1111/j.1740-0929.2010.00752.x