Jen Chung LiaoShiau Tzu TzengGun KeorochanaKwang Bok LeeJared S. JohnsonYuichiro MorishitaSamuel S. MurrayJeffrey C. WangChang Gung UniversityDavid Geffen School of Medicine at UCLATzu Chi UniversityMahidol UniversityChonbuk National University, School of MedicineVA Medical Center2018-05-032018-05-032011-05-01Journal of Orthopaedic Research. Vol.29, No.5 (2011), 753-7591554527X073602662-s2.0-79952779064https://repository.li.mahidol.ac.th/handle/20.500.14594/12530Bone morphogenetic binding peptide (BBP) is an 18.5 kDa fragment of a bone matrix protein peptide. A rat femoral defect model was used to test the effect of BBP combined with recombinant human bone morphogenetic protein-7 (rhBMP-7) to induced bone healing. Two doses of BBP (500 and 1000 μg) were tested with two doses of rhBMP-7 (2 and 5 μg), and the results were c ompared with a positive control (10 μg rhBMP-7). Bone healing was evaluated by radiology, manual palpation, microcomputed tomography, and histology. The high dose of 10 μg of rhBMP-7 resulted in a consistent 100% bone union rate and a mature histological appearance on histology, and was used as a positive control. When 1000 μg of BBP was combined with lower doses of BMP-7 (2 μg rhBMP-7 or 5 μg rhBMP-7) significant differences were seen in radiographic scores, manual palpation, and bone volume, when compared to 2 μg rhBMP-7 or 5 μg rhBMP-7 alone. The combination of 1000 μg of BBP and 5 μg rhBMP-7 also achieved 100% fusion rate, induced a larger amount of bone formation, and yielded similar maturity of bone marrow when compared with the high dosage 10 μg rhBMP-7 group. This study demonstrated that when combined together, BBP can enhance the bone healing of rhBMP-7. Improved healing imparted by the addition of BBP may result in lesser amounts of rhBMP-7 needed to achieve union in the clinical setting. Copyright © 2010 Orthopaedic Research Society.Mahidol UniversityMedicineArticleSCOPUS10.1002/jor.21252