Pranee InprakhonNatcha WongthongdeeTaweechai AmornsakchaiThunyarat PongtharankulPanya SunintaboonLars O. WiemannAlain DurandVolker SieberMahidol UniversityFraunhofer Institute for Interfacial Engineering and Biotechnology IGBLaboratoire de Chimie Physique MacromoléculaireUniversité de Lorraine2018-12-212019-03-142018-12-212019-03-142017-10-10Journal of Biotechnology. Vol.259, (2017), 182-19018734863016816562-s2.0-85026344609https://repository.li.mahidol.ac.th/handle/20.500.14594/41747© 2017 Elsevier B.V. Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7 M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2 M sucrose monoester within 1 h at 50 °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1016/j.jbiotec.2017.07.021