S. CheevadhanarakG. SaundersD. V. RennoT. W. FlegelG. HoltUniversity of WestminsterMahidol University2018-08-102018-08-101991-01-01Journal of Biotechnology. Vol.19, No.1 (1991), 117-122016816562-s2.0-0025736709https://repository.li.mahidol.ac.th/handle/20.500.14594/22005Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200 μg ml-1phleomycin. A frequency of 3 to 7 transformants per 106viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites. © 1991.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyImmunology and MicrobiologyArticleSCOPUS10.1016/0168-1656(91)90079-B