Uta ErdbrüggerCharles J. BlijdorpIrene V. BijnsdorpFrancesc E. BorràsDylan BurgerBenedetta BussolatiJames Brian ByrdAled ClaytonJames W. DearJuan M. Falcón-PérezCristina GrangeAndrew F. HillHarry HolthöferEwout J. HoornGuido JensterConnie R. JimenezKerstin JunkerJohn KleinMark A. KnepperErik H. KoritzinskyJames M. LutherMetka LenassiJanne LeivoInge MertensLuca MusanteEline OeyenMaija PuhkaMartin E. van RoyenCatherine SánchezCarolina SoekmadjiVisith ThongboonkerdVolkert van SteijnGerald VerhaeghJason P. WebberKenneth WitwerPeter S.T. YuenLei ZhengAlicia LlorenteElena S. Martens-UzunovaSiriraj HospitalSwansea University Medical SchoolDepartment of Chemical Engineering, TU DelftEdinburgh Medical SchoolErasmus MC Cancer InstituteCIC BioGUNEOslo UniversitetssykehusCardiff University School of MedicineOsloMet – storbyuniversitetetUniversity of Michigan Medical SchoolErasmus MCVanderbilt University Medical CenterUniversità degli Studi di Torino, Scuola di MedicinaUniversity of Virginia Health SystemHospital Universitari Germans Trias i PujolQIMR Berghofer Medical Research InstituteUniversitätsklinikum des Saarlandes Medizinische Fakultät der Universität des SaarlandesUniversity of Louisville Health Sciences CenterUniverza v Ljubljani Medicinska FakultetaClinica Las CondesUniversiteit AntwerpenUniversità degli Studi di TorinoNational Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)La Trobe UniversityTurun yliopistoNational Heart, Lung, and Blood Institute (NHLBI)Universitätsklinikum Hamburg-EppendorfHelsingin YliopistoSouthern Medical UniversityRadboud University Medical CenterOttawa Hospital Research InstituteAmsterdam UMC - Free University AmsterdamJohns Hopkins School of Medicine2022-08-042022-08-042021-05-01Journal of Extracellular Vesicles. Vol.10, No.7 (2021)200130782-s2.0-85106448800https://repository.li.mahidol.ac.th/handle/20.500.14594/76188Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyMedicineArticleSCOPUS10.1002/jev2.12093