Samon TrungkathanDuangporn PolpanichSrung SmanmooPramuan TangboriboonratMahidol UniversityThailand National Nanotechnology CenterThailand National Center for Genetic Engineering and Biotechnology2018-11-092018-11-092014-03-15Journal of Applied Polymer Science. Vol.131, No.6 (2014)10974628002189952-s2.0-84896548681https://repository.li.mahidol.ac.th/handle/20.500.14594/33627Mannose-rhodamine (Rh) conjugate (80% yield) was synthesized in a one-pot reaction and immobilized onto magnetic polymeric nanoparticles (MPNP; 43% magnetic content) of poly(styrene/divinyl benzene/acrylic acid). The resulting nanoparticles contained MPNP as a substrate, mannose as an E. coli receptor and Rh as a fluorescent signaling unit. TEM imaging clearly demonstrated that multiple mannose-Rh MPNPs could be captured by E. coli strain ORN178. The fluorescent signal from captured nanoparticles was emitted at 580 nm. These results indicate that mannose-Rh MPNP offers a simple and rapid strategy for bacterial detection. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014, 131, 40012. Copyright © 2013 Wiley Periodicals, Inc.Mahidol UniversityChemistryMaterials ScienceArticleSCOPUS10.1002/app.40012