Prakan ThanasrisuebwongSirichai KiattavorncharoenRudee SuraritChareerut PhruksaniyomNisarat RuangsawasdiMahidol University2020-08-252020-08-252020-01-01International Journal of Molecular Sciences. Vol.21, No.14 (2020), 1-1214220067166165962-s2.0-85088271042https://repository.li.mahidol.ac.th/handle/20.500.14594/57740© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The biological benefits of using two fractions derived from injectable platelet-rich fibrin (i-PRF) in bone regeneration remain unclear. Thus, the current study examined two fractionation protocols producing yellow i-PRF and red i-PRF on periodontal ligament stem cells (PDLSCs). The i-PRF samples from five donors were harvested from two different levels, with and without a buffy coat layer, to obtain red and yellow i-PRF, respectively. The PDLSCs were isolated and characterized before their experimental use. The culture medium in each assay was loaded with 20% of the conditioned medium containing the factors released from the red and yellow i-PRF. Cell proliferation and cell migration were determined with an MTT and trans-well assay, respectively. Osteogenic differentiation was investigated using alkaline phosphatase and Alizarin red staining. The efficiency of both i-PRFs was statistically compared. We found that the factors released from the red i-PRF had a greater effect on cell proliferation and cell migration. Moreover, the factors released from the yellow i-PRF stimulated PDLSC osteogenic differentiation earlier compared with the red i-PRF. These data suggest that the red i-PRF might be suitable for using in bone regeneration because it induced the mobilization and growth of bone regenerative cells without inducing premature mineralization.Mahidol UniversityBiochemistry, Genetics and Molecular BiologyChemical EngineeringChemistryComputer ScienceArticleSCOPUS10.3390/ijms21145153