Supanee HirunkanokpunJonathan O. CarlsonPattamaporn KittayapongMahidol UniversityColorado State University2018-07-122018-07-122008-05-01American Journal of Tropical Medicine and Hygiene. Vol.78, No.5 (2008), 784-790000296372-s2.0-45349100247https://repository.li.mahidol.ac.th/handle/20.500.14594/19330Four mosquito densovirus strains were assayed for mortality and infectivity against Aedes aegypti larvae from different geographic regions. The viral titers were quantified by real-time PCR using TaqMan technology. First-instar larvae were exposed to the same titer of each densovirus strain for 48 hours. All strains of densoviruses exhibited larvicidal activity and caused more than 80% mortality and infectivity in the three mosquito strains. Aa/DNV-exposed larvae had the highest mortality rate. The mean time to death of Aa/DNV-exposed larvae was shorter than other DNVs-exposed larvae. We can conclude that different densovirus strains exhibit some variations in their pathogenicity to different populations of Ae. aegypti mosquitoes. A few mosquitoes from Chachoengsao and Bangkok exposed to AeDNV and AThDNV survived to the adult stage to lay eggs and showed 22% to 50% vertical transmission in the F1 generation. Phylogenetic analysis of four densovirus strains indicated that mosquito densoviruses are separated into two distinct clades. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene.Mahidol UniversityImmunology and MicrobiologyMedicineArticleSCOPUS